Xenogen ivis 200 manual

Xenogen ivis 100 in vivo imaging system university imaging. Power source charge the battery pack in temperatures between approxi mately 0 c and 40 c 32 f and 104 f. Ivis automatically compensates for the changes in light collection so these measurements can be compared to those collected earlier and later in the experiment. Ivis imaging system 3d, 200, 100, and 50 series models living image software for digital image processing and analysis on windows and macintosh computer platforms light producing cells and microorganisms, including bacteria and human tumor cells tagged to emit light. Accelerating research with ivis lumina s5 and x5 highthroughput systems.

We found that green fluorescent proteinexpressing cells 100 x 10 6 were not detectable in a mouse cadaver phantom experiment. Ivis 200 ivis 200 series components thermoelectrically cooled ccd camera with water chiller, imaging chamber with fully integrated design, customdesigned optics, singleview 3d reconstruction, acquisition computer, high resolution monitor, integrated fluorescence capability, integrated anesthesia. Ivis 200 5 fluorescent imaging in the ivis imaging system 200 series emission filter wheel excitation filter wheel excitation light light source filter set label background passband nm excitation passband nm emission passband nm dyes, fluorescent proteins, and quantum dots 1 green 410 440 445 490 515 575 gfp, egfp, fitc. Its never been easier or faster to get robust dataand answerson anatomical and molecular aspects of disease. Please log off from the computer after each use to prevent your group from being overcharged for your time. Please do not turn off the xenogen system computer or ivis system components. Hi tomi, 1 two ip injections of luciferin are given in case of a missed injection, although a partial dose may not give you a satisfactory image in which case you will need to reimage the animal. The filter set of four covers the excitation range of 445750 nm and emission of 500875 nm. It relies on patented optical imaging technologies to facilitate noninvasive longitudinal monitoring of disease progression, cell trafficking and gene expression. Comparatively inexpensive technique for noninvasive study of molecular targets inside the body of the living animals.

Small animal imaging core licor pearl imager contact. When generating an image series or imaging a group of animals, perform instrument subtraction first and only once. Xenogen corporation 860 atlantic avenue alameda, california 94501 usa phone 510 2916100. The driver may be included in your os or you may not need a driver. Transcending epithelial and intracellular biological. The system uses leading optical technology for preclinical imaging research and development ideal for noninvasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals. Turn on the evacuation pump using the switch on the front panel. The vixia gx10 is canons first 4k uhd consumer camcorder.

Novel intraperitoneal treatment with nonthermal plasma. The ivis spectrum in vivo imaging system combines 2d optical and 3d optical tomography in one platform. Characterization of thyroid cancer cell lines in murine. In vivo imaging systems ivis detection of a neuroinvasive. Xenogen mdamb231lucd3h1 bioware cell line product sheet page 3 of 4 discovery in the living organism xenogencorporationin vivobiophotonic imagingtechnologies figure 4. The mixture was introduced into the left lungs of mice via an orthotopic injection. Materials and methods mammalian expression vector preparations, transfections, and stable clone selection the coding sequences of mcherry, mplum, and tdtomato were kindly supplied by roger tsien as plasmids. It is ideally used to monitor disease progression, cell trafficking and gene expression patterns in live mice.

Bioluminescent imaging was performed, as previously described, using the xenogen ivis 200 imaging system luo et al. Preclinical imaging facility, hosted at ceinge ibb cms. The systems lighttight imaging chamber has a heated platform and portals for delivery of gaseous anesthesia to five mice or two rats at a time. Perkinelmer caliper ivis spectrum hardware manual pdf download. The ivis lumina s5 2d optical imaging system combines high sensitivity optical and patented spectral unmixing capabilities in a highthroughput system. Offering an outstanding combination of features and functionality in a compact and portable design, the vixia gx10 delivers refined video quality with stunning detail to video enthusiasts and aspiring filmmakers. Its capable of imaging bioluminescence and fluorescence. I make royalty free music that you can use in your gamesvideosflashes, etc. Important confirm that all of the imaging parameters in th e ivis system control panel are set to the same values used to acquire the image. The ivis spectrum is the most versatile and advanced in vivo imaging system available on the market today. Product sheet xenogen bioware cell line mdamb231lucd3h1. Noninvasive optical tracking of red fluorescent protein.

Be sure to fill out the log binder to accurately record your user time, as well as logging on and off the system. Adjust camera settings to obtain a signal level of 600 to 60,000 counts. It uses xenogens novel patented optical imaging technology to facilitate noninvasive longitudinal monitoring of disease progression, cell trafficking, and gene expression patterns in living animals. The xenogen ivis 100 from caliper life sciences can be used for both bioluminescence and fluorescence. As shown, mice were imaged weekly from dorsal and ventral views for 10 weeks.

Ivis spectrum in vivo imaging system university imaging centers. Xenogen ivis200 central microscopy research facility. The ivis imaging system 200 series is xenogen s first fully integrated system, combining all imaging components into a 25x34inch portable footprint. Mdamb231lucd3h1 cells 1x105 are injected into the left ventricle of female nude mice n. Xenogen ivis200 user manual read living image software manualliving lmage softwareversion 2. Select a method and use it consistently throughout your study to avoid any.

The ivis lumina xr should be operated only by personnel who have been trained in radiation safety and the operation and safety instructions contained in this manual. Follistatinlike protein 1 inhibits lung cancer metastasis by. Mdamb231lucd3h1 cells 1x105 are injected into the left ventricle of female nude mice n5. Integrated into the system are both a bioluminescence system and a fluorescence system and the capability to easily switch between modalities. The perkinelmer ivis imaging system 200 series is an advanced singleview 3d optical imaging system designed to improve quantitative outcomes of in vivo imaging. The system allows for easy animal handling and basic maintenance is minimal.

A single injection of luciferin can be administered and the animal may be conscious or unconscious. The xenogen ivis200 system is a highsensitivity, low noise, in vivo imaging technology platform that allows noninvasive visualization and tracking of cellular and genetic activity within a living organism, in real time. Jul 14, 2009 the ivis 200 series performed as advertised. Core descriptions for grants university of maryland.

In vivo bioluminescent imaging of mammary tumors using. Optical imaging utilizes a variety of labeled molecules. If you are using a battery pack recommended by canon for use with this camcorder, there may be a problem with the battery pack. The analysis was performed using the xenogen ivis200 system xenogen. For orthotopic experiments, mice were imaged ventrally with the xenogen ivis 200 imaging system, and for intracardiac injection experiments, both dorsal and ventral images were obtained. In vivo bioluminescent imaging of mammary tumors using ivis. This system allows researchers to use realtime, noninvasive imaging to monitor and record cellular and genetic activity in vivo. Turn on the main power switch and the camera on the backside of the system 3. Xenogens vivovision systems utilize biophotonic imaging and serve to expedite drug discovery and development, thereby minimizing upfront costs and significantly. The ivis ccd camera has a signal range of 0 to 65535 analog to digital counts 216. Xenogen ivis 200 manual fmt 4000 i s a preclinical tomographic 3d fluorescence imaging system, and is useful for a range of biomedical applications in the fields of oncology, inflammation and cardiovascular diseases.

Xenogens ivis 200 series and living image software is the property of its rightful owner. The system uses a novel xenogen technology in vivobiophotonic imagingto allow researchers to use realtime imaging to monitor and record cellular and genetic activity within a living organism. Cibr is a novel center that provides the infrastructure and oversight of a collection of 26 scientific core facilities that provide stateoftheart technologies, hightech instrumentation, and expertise to support biomedical research, clinical practice and health care to principal investigators in the state of maryland and the region. The xenogen ivis spectrum is an advanced multimodal imaging system used for highsensitivity in vivo imaging of fluorescence and bioluminescence with 3d diffuse tomographic reconstruction functions. Anaesthetised mice were injected with 75 mgkg dluciferin caliper life sciences, hopkinton, ma, usa to acquire images using the xenogen.

The ivis 100 imaging system is a highly sensitive and large field of view imaging system that offers users the flexibility to image bioluminescent reporters both in vivo and in vitro. If so, share your ppt presentation slides online with. The xenogen xgi8 gas anesthesia system is designed to work with the ivis imaging system, a technology from xenogen that allows researchers to use realtime in vivo imaging to monitor and record cellular and genetic. Ivis spectrum in vivo imaging system university imaging.

Includes integrated fluorescence and single view 3d capabilities. If you want to do fluorescent images turn on the schott dcriii besides the monitor 5. The living image software supports hardware for ivis imaging system 50, 100, 200, 3d series and lumina see table 1. Mice were anesthetised using isoflurane, and imaged using a three min exposure. An optimized set of high efficiency filters and spectral unmixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. There is no driver for the os version you selected. Turn on the computer windows password is password 4. In our observations, the collected bioluminescent signals were sustainable and strong, and the proprietary software gives a wide range of flexibility for the quantification of photons flux. Xgi8 gas anesthesia system contact information please call 1.

Follistatinlike protein 1 inhibits lung cancer metastasis. We have evaluated the use of the xenogen ivis 200 imaging system for realtime fluorescence proteinbased optical imaging of metastatic progression in live animals. Core descriptions for grants university of maryland school. The fluorescence equipment enables the user to convenientlyswitch between bioluminescent and fluorescent imaging applications.